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Study of Biomolecules with Inhibitory Activity on Helicobacter pylori in Colombian Patients with High Risk of Gastric Cancer
Estudio de Biomoléculas con Actividad Inhibitoria sobre Helicobacter pylori en Pacientes Colombianos con Alto Riesgo de Cáncer Gástrico
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Burbano-Vasquez, D. M., Lopez Miramag, C. D., Maigual Enriquez, L. C. ., Legarda Nastul, Y. B. ., Arango Bedoya, O. ., Hurtado Benavides, A. M., & Pazos Moncayo , A. J. . (2025). Study of Biomolecules with Inhibitory Activity on Helicobacter pylori in Colombian Patients with High Risk of Gastric Cancer. NOVA, 23(44). https://doi.org/10.22490/
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Lizeth Camila Maigual Enriquez
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Introduction: Gastric cancer in Nariño represents a significant public health problem due to its high incidence and mortality rates. Eradicating Helicobacter pylori is a key strategy for preventing this disease, but the failure of some treatments has prompted the search for alternatives, such as the use of natural compounds. Among these, Brassica oleracea var. Itálica and Cannabis sativa extracts rich in cannabidiol stand out. Objective: To evaluate the in vitroinhibitory activity of broccoli and Cannabis sativa extracts rich in cannabidiol on the growth of Helicobacter pyloriisolated from patients with gastric disease in Nariño. Methodology: Helicobacter pyloriwas isolated and purified from gastric biopsies of patients from the Department of Nariño. Brassica oleraceavar. Itálica extracts, with high sulforaphane content, were obtained using rotaevaporation and lyophilization techniques. The Cannabis sativa extract was obtained using supercritical CO₂ extraction. The inhibitory activity of the extracts was evaluated using the agar dilution method, colony-forming units (CFU) were counted using ImageJ software, and the minimum inhibitory concentration (MIC) for each isolate was determined. Results:The MIC of Brassica oleracea var. Itálica extracts with high sulforaphane content was 120 µg/mL. The MIC of the Cannabis sativa extract was 55 µg/mL. Conclusion: Brassica oleracea var. Itálica and Cannabis sativaextracts rich in cannabidiol exhibit an inhibitory effect on the in vitro growth of Helicobacter pyloriisolated from patients in Nariño, with the Cannabis sativa extract being the most effective.
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